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XMAP215 promotes microtubule nucleation independently as a polymerase of nucleation intermediates. Across a range of polymerase activities, XMAP215 and γ-tubulin function additively, indicating independent modes of action. Any synergistic behavior must arise from unique interactions made in the context of the γ-TuRC.

XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions