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Using polyacrylamide hydrogels with either physiological (≈3 kPa soft) or pathological (≈40 kPa stiff) tissue stiffness, we have discovered an inverse relationship between matrix stiffness and paraspeckle abundance in several cancer cell lines including triple negative breast cancer MDA-MB-231.

Matrix stiffness-sensitive long noncoding RNA NEAT1 seeded paraspeckles in cancer cells