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The translocation of CCTα, the rate-limiting enzyme for phosphatidylcholine synthesis, to nuclear membranes and nuclear lipid droplets results in reversible dephosphorylation of S319 and sustained phosphorylation of Y359+S362. Independent regulation of these phosphosites in the P-domain of CCTα is required for activation on nuclear membranes.

Differential dephosphorylation of CTP:phosphocholine cytidylyltransferase upon translocation to nuclear membranes and lipid droplets