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Here we introduce an optical flow motion estimation approach to study microtubule (MT) orientation in the Drosophila oocyte, a cell displaying substantial cytoplasmic streaming. We show that MT polarity is affected by the regime of these flows and, furthermore, that the presence of flows is necessary for MTs to adopt their proper polarity.

Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in theDrosophilaoocyte