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The GTP-bound form of ADP-ribosylation factor-like 4D (Arl4D) small GTPase localizes to the centrosome and interacts with microtubule (MT) end-binding 1 (EB1) protein. Arl4D-EB1 interaction promotes EB1 centrosomal recruitment and increases the association of EB1 and p150 subunit of dynactin, thereby contributing to the nucleation of MTs at the centrosome.

Arl4D-EB1 interaction promotes centrosomal recruitment of EB1 and microtubule growth