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The wealth of information from genetic and more recently genomic studies of the budding yeast has been staggering. However, the small size of the organism has limited traditional cytological approaches. Thus there is a disparity in our detailed understanding of the genetic control of mitosis, for instance, relative to the morphology of mitosis. The application of time-lapse high-resolution digital-enhanced differential interference contrast (DE-DIC) and multimode fluoresence microscopy to studying yeast cell division has revealed a very dynamic process that reflects the interplay among microtubule dynamics, microtubule-based motor proteins, and positional determinants in the mother and bud (Salmon et al., 1998a ,b ; Shaw et al., 1997a ,b ).

Nuclear and Spindle Dynamics in Budding Yeast