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Accurate analysis of dinucleotide redox cofactors nicotinamide adenine dinucleotide phosphate reduced (NADPH), nicotinamide adenine dinucleotide phosphate (NADP + ), nicotinamide adenine dinucleotide reduced (NADH), and nicotinamide adenine dinucleotide (NAD + ) from biological samples is important to understanding cellular redox homeostasis. In this study, we aimed to develop a simple protocol for quenching metabolism and extracting NADPH that avoids interconversion among the reduced forms and the oxidized forms.

Extraction and Quantitation of Nicotinamide Adenine Dinucleotide Redox Cofactors