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Objective: Previous studies have demonstrated that laserpuncture has a positive effect on longitudinal bone growth within varying parameters. The current authors hypothesized that laserpuncture affects serum concentration of insulinlike growth factor 1 (IGF-1), a growth factor that influences growth plates, and ghrelin, a growth hormone (GH) secretagogue. The goal of this research was to investigate if laserpuncture could increase serum concentrations of IGF-1 and ghrelin in adolescent rats. Materials and Methods: This was an experimental study with a post-test only and a controlled group design. The study was performed in a laboratory on 40 male Wistar rats, age ±3 weeks and weighing &gt;40 g. The rats were divided randomly into 2 groups, A and B, each receiving laserpuncture for 10 days and 15 days, respectively. There were 4 subgroups in each group: control (C); GV 20; ST 36; and GV 20 + ST 36. The rats were stimulated by laserpuncture with a KX Laser GX-2000B (Kangxing), a semiconductor-based low-level laser therapy (LLLT) device emitting a cold red laser (635-680 nm/5 mW) for 60 seconds (0.3 J/cm 2 ) The length of each rat's lower limb was measured using a caliper; IGF-1 and ghrelin concentrations were assessed using enzyme-linked immunosorbent assays. Results: A marked increase in IGF-1 concentration was observed in the ST 36 and GV 20 + ST 36 subgroups after 15 days, compared to the C subgroup ( P  = 0.001 and P  = 0.002, respectively). The GV 20 subgroup had a lower IGF-1 concentration than the C subgroup ( P  = 0.06) at both 10 and 15 days. Laserpuncture did not influence ghrelin concentrations significantly, compared to the C subgroups. Conclusions: Laserpuncture administered to both the ST 36 and GV 20 + ST 36 subgroups with a power density of 0.3 J/cm 2 increased serum concentrations of IGF-1 in adolescent rats.

Laserpuncture Increases Serum Concentration of Insulin-Like Growth Factor–1 in Adolescent Rats