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In this work porous microparticles of calcium carbonate were synthesized with bovine serum albumin fluorescein isothiocyanate conjugate (BSA-FITC) and dexamethasone, and then used for encapsulation in polymer microcapsules by means of layer-by-layer assembly (LbL). The properties of the obtained microcapsules were characterized by scanning electron microscopy, dynamic light scattering, infrared-, ultravioletand visible spectroscopy. According to the performed DLS measurements, an average hydrodynamic diameter ranged from 4 to 8 μm and zeta-potential for all types of capsules was determined as -18 and -21 mV. BSA-FITC was encapsulated using this approach yielded a loading efficiency of 49 % protein. This value calculated for dexamethasone was of 38%. The microcapsules filled with an encapsulated drug may find applications in the field of biotechnology, biochemistry, and medicine.

Determination of the properties and loading efficiency of encapsulated BSA-FITC and dexamethasone for drug delivery systems