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This study was conducted in tissue culture laboratories, Faculty of agriculture, University of Kufa in 2020. The experiment included studying the effect of two factors: type of light: (mixture spectra of red and blue LED R16 : B2), (Fluorescent : normal white light) in interaction with different concentrations of cysteine( 0, 50, and 100 mg.L -1 ). A completely randomized design (C.R.D) were used with two factor and three replicates. The combined spectra (LED R16:B2) increased the active compounds Di-allyldisulfide and Vinyldithiin (131.33,121.41μg.g -1  F.W. ) respectively. Cysteine (100 mg.L -1 ) was significantly superior compare to other treatments in producing Di-allyldisulfide and Vinyldithiin content (158.87, 144.35 μg.g -1  F.W) respectively. Results also indicated that the interaction treatment between (LED R16:B2) and (Cysteine 100 mg.L -1 ) gave significant increase in Di-allyldisulfide and Vinyldithiin content (184.28, 146.07μg.g -1  F.W) respectively. While the tretment (Fluorescent W) and (Cysteine 50 mg.L -1 ) gave significant increase in Allicin content (195.38 μg.g -1  F.W).

Effect of Cysteine in Stimulating Some Active Compounds in Garlic Callus Under Different Light Spectra in Vitro