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The agricultural residue can be significantly transformed to both renewable energy and high value-added chemicals using biorefinery concept. Biological pre-treatment of lignocellulosic biomass and ethanol production from cacao pod husk can be done within a single unit-operation using a single microorganism white rot  Phlebia sp. MG-60 . The objective of this study was to identify the potential of white rot  Phlebia sp. MG-60  to produce ethanol and aromatic compounds under consolidated biological processing (CBP). The effect of different concentration of MnSO 4  on detecting of cellulose, hemicellulose, lignin and MnP activity were measured on 0, 14 and 28 days. The selected best concentration of MnSO 4  was then used for detecting of potential aromatic compound released and the yield of ethanol. By adding the inducer (MnSO 4 ), the results revealed that the addition of MnSO 4  could escalate the activity of  manganese peroxidase  (MnP) from  Phlebia sp. MG-60  in order to depolymerize lignocellulose. The yield of ethanol produced during the bioconversion process was 2g/L. Furthermore, several aromatic compounds such as 2, 3-Dimethylphenol, trans-cinamic acid, caffeic acid, and vanillin were potentially obtained from cacao pod husk incubated by the fungus for 28 days.

The identification of ethanol and aromatic compounds from delignification of cacao pod husk using Phlebia sp.MG-60