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The objective of research is determined the virulence of different concentrations of SlNPV-JTM 97C with kaolin as carrier material on  S. litura and T. laeviceps  mortality  on soybean . The Research was conducted in Plant Protection Laboratory of State polytechnic of Jember, from June to September 2020. The lethal concentration (LC 50  and LC 95 ) SlNPVusing Polo Plus 1.0, with the IRAC version 3, method number 008. The  S. litura  mortality test used Completely Randomind Design with 5 treatments and 3 replications. The treatments were contamination of larvae diet by SlNPV with control (the third instar larvae without contamination SlNPV), 0,1%, 0,2%, 0,3% and 0,5%. These concentrations are equivalent to the concentrations of 0.1.2x 10 8  PIBs/g, 2.4x 10 8  PIBs/g, 3.6 x 10 8  PIBs/g and 6.0 x 10 8  PIBs/g, respectively. Each test was repeated three times. Mortality percentage of larvae were observed as experiment data. Those data were analyzed by Analysis of Variant and Least Significant Difference (LSD) 5%. SlNPV virulence test against  T. laeviceps  using a concentration that can cause  S. litura  mortality ≥ 70%. The results of the research indicated that LC 50  of SlNPV with kaolin as carrier against  S. litura  3 rd  instar was 0.2%, LC 95  was 0.5%, effective concentration of SlNPV to control  S. litura  3 rd  instar was 0.3% to 0.5%, SlNPV concentration at 0.3% to 0.5%, non-toxic to  T. laeviceps .

iop conference series. earth and environmental science

Virulence of Spodoptera Litura Nuclear Polyhedrosis Virus (SLNPV) with kaolin as carrier material on spodoptera litura and tetragonula laeviceps on soybean