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The article discusses the results of screening strains of probiotic bacteria by their ability to metabolize cholesterol during development on different nutrient media. Cholesterol content was determined by the method of Zlatkis-Zack. The results on the possibility of creating starter cultures from proven strains capable of lowering cholesterol in vitro and taking into account technological properties were presented. Four starter cultures were created, which included microorganisms belonging to two genera: Lactobacillus, Bifidobacterium (strains were deposited). The created starter cultures showed a higher activity for cholesterol reduction than each strain separately. To confirm the revealed effect, studies of starter cultures were carried out in vivo on the SHK line both sexes white mice. The research results showed that when using the created starter cultures in the nutrition of white mice, against a background of elevated cholesterol, there was a decrease in the concentration of total cholesterol in the animal blood by 38.5 % maximum, compared with mice fed with high cholesterol but without starter cultures. The obtained research results allow us to draw to a conclusion about the possibility of participation of the created starter cultures in cholesterol metabolism and recommend them for use in biotechnology of functional foods.

Biotechnology of starting culture capable of cholesterol metabolism