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Tofu is one of favourite daily meal in Indonesia. It is produced freshly in some small to large-scale industries, and generates a large volume of wastewater. Generally, untreated wastewater from tofu industries in Indonesia is still directly disposed to the environment that can cause severe pollution in water resources. Tofu wastewater contains very high organic compounds and nutrients, but it has an acidic pH. The possibility of utilizing tofu wastewater as a cultivation medium for microalgae is intriguing. The objective of this research was to utilize tofu wastewater as a cultivation medium for  Chlorella vulgaris  and  Arthrospira platensis . Cultivation of microalgae was conducted for ten days using 3, 5, and 10 % of diluted tofu wastewater. For comparison, the tofu wastewater anaerobic digestion effluent (TW-ADE) was used as the medium. Walne medium was used as a positive control, and distilled water was used as a negative control. Both microalgae showed the highest biomass and protein production was in Walne medium (positive control), followed in 5% tofu wastewater medium for  C. vulgaris , and 3 % tofu wastewater medium for  A. platensis . The highest chlorophylls content of  C. vulgaris  and  A. platensis  was in 5 % tofu wastewater and Walne medium, respectively. The most top carotenoid content of  C. vulgaris  and  A. platensis  was in 5 % and 10 % tofu wastewater medium, respectively. On the other hand, biomass, chlorophylls, carotenoids, and protein content of both microalgae in TW-ADE medium had the lowest result. Cultivation of  C. vulgaris  and  A. platensis  in  tofu waste medium offer a wide range of environmental and economic benefits, as a sustainable strategy to treat wastewater and produce high-value products from microalgae.

Utilization of tofu wastewater as a cultivation medium for Chlorella vulgaris and Arthrospira platensis