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The dead time behavior from several inert gas and mathematical methods under temperature and column variation have been studied using randomized block design. Dead time of inert gas and mathematical dead time calculated using n-alkane homologous series that measurement with gas chromatography at temperatures of 60, 90 and 120°C and columns of PS-255, OV-11, CPSIL-5CB, HP -5 and HP-INNOWAX were used as a set data in randomized block design. The results showed that temperature variation affects all dead time values, either for inert gas dead time or mathematical method, except for Parcher method. Different column types between capillary and packed column exhibits a significant effect. For capillary column, all dead time values, either for inert gas dead time or mathematical method is not affected by polarity variation, but they are affected by the column length. Meanwhile for packed column, column length does not affect the mathematical dead time of Ne and He gases, but it affects the dead time of O 2  and Ar gases. Parcher method exhibits the best result with a robust dead time, without significant effect in statistics by the change of temperature and column.

iop conference series. earth and environmental science

Analysis of temperature and column variation in gas chromatography to dead time of inert gas and n-alkane homologous series using randomized block design