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Climate change made rain fall unpredictable leading to sudden drought stress during crop cultivation period. Drought stress affects the crop growth and development, especially during the seedling period. This research aims to evaluate the performance of three local rice seedling under drought stress induced by PEG 6000. Three local rice cultivars were germinated and then divided into five groups. In the 3 d after germination, each group were treated in vitro with either 0 %, 10 %, 15 %, 20 % or 25 % PEG 6000 solution for 10 d in vitro. Drought stress was continued for 14 d ex vitro in rice treated with 0 % and 25 % PEG. Osmotic stress induced by PEG significantly reduced plant growth and total chlorophyll content in the leaf. In vitro study showed rice seedling growth reduction was increased when the PEG 6000 increased. Ex vitro study with application of 25 % PEG showed cv. Telaseh more tolerant to drough stress at the seedling stage compare to cv.. Mayas Kuning and cv. Mayas Putih. Additionally, drought stress induced increasing of soluble sugar in the leaf of all rice cultivars. This might indicate that soluble sugar plays a role as osmoprotectant under drought stress in those three cultivars.

The performance of three local rice (Oryza sativa L.) cultivar from East Kalimantan - Indonesia under drought stress at early seedling stage