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The research study elucidates the dielectric parameters of both diabetic and nondiabetic human blood relatives to frequency which are paramount for blood study and functional therapeutic mechanisms using He-Ne laser irradiation. In the work, the Agilent 4294A impedance analyser within frequency range 40 Hz – 30 MHz and designed cuvette cells with two electrodes each were used for the analysis of 48 human blood samples (24 diabetic blood of glucose level 9.28 mmol/L and 24 non-diabetic blood) collected through venepuncture. Irradiations were done with 532 nm wavelength He-Ne laser of power output of 60 mW for 5, 10, 15 and 20 minutes’ durations. The studied dielectric parameters (dielectric constant, dielectric loss and electric conductivity) demonstrated significantly high values in the control diabetic blood with low impedance level compared to the control non-diabetic blood which demonstrated higher impedance level. The irradiated diabetic blood shows reduced values of the dielectric parameters after irradiation exposure durations of 5 and 10 minutes with significant increase in impedance level. While the dielectric parameters significantly increased when exposed diabetic blood for 15 and 20 minutes became crenated therefore abruptly experienced a dropped in the impedance level. The observed significant variation in dielectric parameters and impedance levels of both blood could be recognised as the result of cellular morphology and the physiological conditions of erythrocytes membranes and other constituents which characterizes its electric response. The irradiated diabetic blood demonstrates positive biostimulation effects due to the enhance stimulation ability of the He-Ne laser irradiation as a safe and effective therapeutic modality for diabetes mellitus.

Frequency characterization of dielectric parameters and impedance of irradiated diabetic blood using He-Ne laser and non-diabetic human blood samples