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In this research, an investigation on the influence of particle size variation in the authentication of peaberry and civet ground roasted coffee using UV-visible spectroscopy and two different compensation methods was conducted. A total of 398 samples of peaberry and civet coffee with two particle sizes of 1680 μm dan 297 μm (mesh 12 and mesh 50) were prepared. The all coffee samples were extracted using hot distilled water. The extracted aqueous coffee samples were pipetted into 10 mm of cuvette and the spectral data was acquired using a UV-Vis spectrometer in the range of 190-1100 nm. The result of PCA showed that the samples can be clustered based on types of coffee (peaberry and civet) and based on particle size (mesh 12 and mesh 50) using pre-processed spectral data in the range of 250-450 nm. Using the global calibration model of PLS1 and PLS2, the prediction results for prediction sample set at particle size of 1680 μm dan 297 μm (mesh 12 and mesh 50) were excellent with low RMSEP, low SEP and low bias. This result demonstrated the ability of global calibration model (both based on PLS1 and PLS2) to compensate the influence of particle size variation on the authentication of peaberry and civet ground roasted coffee.

journal of physics. conference series

The development of classification model of peaberry and civet ground roasted coffee using UV-visible spectroscopy and two different compensation methods