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Microcrystalline cellulose(MCC) was isolated from corncobs waste. It was activated by NaOH solution with varied concentration in range from 4%, 6%, 8%, 10%, 12%, 14% and 17%. Characterization of physicochemical properties of microcrystalline cellulose (MCC) molecular weight was conducted by using Mass Spectrofotometer (MS).Preconditioning of MS used 20 μl injection volume, 10 μl concentration, acethonitrile: ultra water (50:50) as eluent. The result showed that NaOHconcentration affect to the type of oligosaccharides fragmentationwith an increased abundance. MS spectra for NaOH 4% showed four major peaks at 834 m/z, 623 m/z, 425 m/z, 383 m/z that indicated 2-5 glucose polymers respectively, with an increased abundance withadditional Na+ and K+ ions. NaOH8%, 12%, 14%, 17% indicated the type of oligosaccharide fragmentation with equal three major peaks at 827 m/z, 623 m/z, 425 m/z. The fragmentatition type of NaOH 10% and avicel standard pH 102, indicated 2-5 glucose polymers respectively,with an increased abundance with additional Na+ and K+ ions, there are peak at 827 m/z and 425 m/z, and there is no peak at 623 m/z because there are no Na* and K+ ions.

Characterization and analysis of the molecular weight of corn corbs microcrystalline cellulose (MCC) fiber using mass-spectrometry methods