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In this paper, we have studied, within a discrete WLC model, the spatialconstraints in magnetic tweezers used in single molecule experiments. Twoelements are involved: first, the fixed plastic slab on which is stuck theinitial strand, second, the magnetic bead which pulls (or twists) the attachedmolecule free end. We have shown that the bead surface can be replaced by itstangent plane at the anchoring point, when it is close to the bead south polerelative to the force. We are led to a model with two parallel repulsiveplates: the fixed anchoring plate and a fluctuating plate, simulating the bead,in thermal equilibrium with the system. The bead effect is a slight upper shiftof the elongation, about four times smaller than the similar effect induced bythe fixed plate. This rather unexpected result, has been qualitativelyconfirmed within the soluble Gaussian model. A study of the molecule elongationversus the countour length exhibits a significant non-extensive behaviour. Thecurve for short molecules (with less than 2 kbp) is well fitted by a straightline, with a slope given by the WLC model, but it does not go through theorigin. The non-extensive offset gives a 15% upward shift to the elongation ofa 2 kbp molecule stretched by a 0.3 pN force.Comment: 28 pages, 6 figures An explanatory figure has been added. The  physical interpretation of the results has been made somewhat more  transparen

Spatial constraint corrections to the elasticity of double stranded DNA measured with magnetic tweezers