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The formation mechanism of ice banding in the system of freezing colloidalsuspensions, which is of significance in frost heaving, ice-templating porousmaterials and biological materials, still remains a mystery. Recently, thetheory of secondary nucleation and growth of ice has been proposed to explainthe emergence of a new ice lens. However, this theory has not beenquantitatively examined. Here, we quantitatively measured the initialinterfacial undercooling of a new ice lens and the nucleation undercoolings ofsuspensions. We found that the interfacial undercooling can not satisfy thenucleation undercooling of ice and hence disprove the secondary nucleationmechanism for ice banding.

Can secondary nucleation exist in ice banding of freezing colloidal suspensions?