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Due to its ultrahigh electron transmissivity in a wide electron energy range,molecular impermeability, high electrical conductivity and excellent mechanicalstiffness the suspended graphene membranes appear to be a nearly ideal windowmaterial for in situ (in vivo) environmental electron microscopy of nano- andmesoscopic objects (including bio-medical samples) immersed in liquids and/orin dense gaseous media. In this communication, taking advantage of littlemodification of the graphene transfer protocol on to metallic and SiNsupporting orifices, the reusable environmental cells with exchangeablegraphene windows have been designed. Using colloidal gold nanoparticles (50 nm)dispersed in water as model objects for scanning electron microscopy inliquids, the different imaging conditions through graphene membrane have beentested. The limiting factors for electron microscopy in liquids such aselectron beam induced water radiolysis and damage of graphene membrane at highelectron doses were discussed.

Electron transparent graphene windows for environmental scanning electron microscopy in liquids and dense gases