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per' 0.9 + 0.2 (36) 0.2 kO.1 (13) 1.3 + 0.2 (23) 0.9 + 0.3 (9) 0.9 + 0.2 (27) per+ 0.8 IL 0.1 (31) 0.7 * 0.1 (22) 0.9 k 0.2 (9) 0.7 + 0.2 (11) 0.8 f 0.1 (20) per" 0.8 f 0.1 (46) 0.07 + 0.04 (16) 1.3 + 0.1 (30) 0.9 + 0.1 (12) 0.8 k 0.1 (34) per'; 13.2113.2 1.1 + 0.2 (38) 0.5 + 0.2 (11) I.4 f 0.2 (27) 1.1 ?0.3(11) 1.2 k 0.2 (27) Total 0.9 + 0.07 (151) 0.4 k 0.07 (62) 1.3 + 0.08 (89) 0.9 + 0.1 (43) 0.9 + 0.08 (108) A. In this series of experiments, dye transfer was scored as the number of cells filled l-2 min after the beginning of each 30 s injection; the numbers of injections are in parentheses. These data are from cells whose resting potentials (pre-injection) were c-28 mV. Because anterior and posterior cells exhibit differences in electrical parameters (7), their scores were tabulated separately. In other cells whose resting potentials ranged from -27 mV to -12 mV (n = 23, 9, 6, and 9, respectively, for the 4 genotypes), similar results were obtained: there were no systemic genotypic variations from the average dye transfer score of 3.5 cells. B. In this series of experiments, dye transfer was scored on a rating scale of O-3 by two individuals who were blind as to the genotype, and the two scores for each injection were averaged. The numbers of injections are in parentheses. Larvae were maintained at 25°C in a 12 h light:12 h dark cycle. Data in the 'Early' column are from glands dissected between 1 and 3 h after 'lights-on'; data in the 'Late' column are from glands dissected within 2.5 h of 'lights-off.' Salivary glands were classified as small or large prior to injections.

The Drosophila period Gene and Dye Coupling in Larval Salivary Glands: A Re-evaluation