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Content of eicosapentaenoic acid (EPA, 20 ∶ 5n-3) and docosahexaenoic acid (DHA, 22 ∶ 6n-3) and then-3/n-6 ratio are important indicators of nutritive value of aquatic invertebrates as food for fish. We studied fattyacid (FA) content and composition of 68 zoobenthic species. Benthic invertebrates differed significantly in theircontents of EPA and DHA and n-3/n-6 ratios. The most valuable food for fish were Insecta, especially Ephemeropteraand Trichoptera, Gammaridae, and Turbellaria (Dendrocaelopsis sp.). The invertebrates of low food qualityfor fish were Hirudinea, Mollusca, Oligochaeta, Bryozoa (Plumatella emarginata), and Coleoptera (Dytiscus lapponicus).Our data suggest that the recent and ongoing global replacement of native species, mainly Insecta, byinvaders, mostly represented by Mollusca, Crustacea, Polychaeta, Oligochaeta, Bryozoa, and Hirudinea, probablywill reduce the nutritive value of food sources for benthivorous fish. In addition, some of the taxa studied havepeculiar FAs, which may be useful as their markers in trophic webs. Thus, 20 ∶ 1n-13, 20 ∶ 2n-6, 22 ∶ 5n-3, and22 ∶ 6n-3 are likely to be considered markers of Mollusca, Hirudinea, Turbellaria, and Gammaridae, respectively, fortracing food webs in freshwater ecosystem

Content of polyunsaturated fatty acids essential for fish nutrition in zoobenthos species