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Reference Condition Approach (RCA) predictive models are used to assess a test site against reference sites probabilistically matched based on habitat. These models are the basis of several major national stream bioassessment programs in the UK, Australia, and Canada. In the usual approach to developing predictive models, discriminant function analysis (DFA) is used to assign a test site to a group of matched reference sites. These groups typically are established by classification of a macroinvertebrate assemblage and matched to the habitat attributes in a single-step DFA model. We examined an alternative to standard DFA in which a series of tiered models are used. This tiered method constructs a model for the 1st division in a hierarchical classification, and then develops models for each further step in the hierarchical classification. We examined the method with 3 training and validation data sets. Validation data consisted of data from reference sites and those same sites after they underwent simulated impairment. We compared the tiered approach to the standard approach based on prediction accuracy and Type 1 and Type 2 error rates for each data set. The tiered DFA models were similar to or slightly better than the standard single-step DFA models in correctly matching validation sites to reference groups, but this improvement in accuracy did not necessarily translate into improved bioassessment error rates.

A tiered method for discriminant function analysis models for the Reference Condition Approach: model performance and assessment