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Pseudorabies virus was cultivated in vitro in washed testicle tissue from immune guinea pigs, and evidence was thus procured which indicated that the testicle cells themselves had not become immune to pseudorabies. The rate of multiplication of the virus was considerably greater in control cultures with normal guinea pig testis than in cultures with immune testis. The reason for this fact may be that even by repeated washing the immune tissue could not be completely freed from fluid antibodies, and that such antibodies somewhat inhibited the multiplication of the virus.

MULTIPLICATION IN VITRO OF PSEUDORABIES VIRUS IN THE TESTICLE TISSUE OF IMMUNIZED GUINEA PIGS