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A medium has been developed in which certain cells from the gonads of female silkworms multiply and live for periods of 2 to 3 weeks. In such tissue cultures, strains of silkworm grasserie virus were maintained in successive passages up to the number of ten. The virus multiplied greatly and typical polyhedral bodies formed in the cells of infected cultures.

CULTIVATION OF THE VIRUS OF GRASSERIE IN SILKWORM TISSUE CULTURES