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There is need for a simple and reliable technique for re-embedding and thin sectioning of thicker sections (previously examined with light microscopy) for electron microscopy. Because of the small area and extreme thinness of sections for electron microscopy, the sampling error of sections taken at random from a tissue mass can be very large. Furthermore, even if some of the random sections do contain the area of interest, its orientation may be unsatisfactory. Searching for a particular small area within a large block by thin sectioning is laborious; and methods in which alternating thick and thin sections arc prepared arc tedious, and may not be adequate for a particular purpose. A method of selecting specific cells or areas of cells to be thin sectioned in known orientation is essential. Several authors have described techniques for doing this (Grimley, 1965; Woodcock and Bell, 1967; Reingold et al., 1970; Mogenson, 1971; Campbell and Hermans, I972; and Springer, 1973). All have certain drawbacks: for instance, the techniques of Grimley (1965), Woodcock and Bell (1967), and Mogenson (1971) require specially built equipment; the technique of Reingold et al. (1970) requires special plastic slides; the technique of Campbell and Hermans (1972) requires excessive handling of sections; while the method of Springer (1973) makes photography of thick sections difficult.

A METHOD FOR SEQUENTIAL HIGH RESOLUTION LIGHT AND ELECTRON MICROSCOPY OF SELECTED AREAS OF THE SAME MATERIAL