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HeLa Cells have been found to produce a placental form of alkaline phosphatase (1, 8, 17) which is very similar to the Regan isoenzyme, a carcinoplacental antigen found in cancer patients (7-9) . In order to investigate the nature of gene expression of carcinoplacental proteins, HeLa cells were cloned, and a subline (HeLa TCRC-1), monophenotypic for the Regan isoenzyme was selected . The properties of another line, HeLa TCRC-2 (HeLa 12-2), discovered by Kelly et al . (14) were studied with regard to non-Regan isoenzyme . MATERIALS AND METHODS

CHARACTERIZATION OF TWO HELA SUBLINES: TCRC-1 PRODUCES REGAN ISOENZYME AND TCRC-2, NON-REGAN ISOENZYME