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Actin-like protein has been isolated from cultured since normal cell morphology is disrupted during sympathetic ganglia of chick (6) and mammalian glycerination, the exact localization of actinbrain (18), and accounts for up to 207 of the total HMM complexes in axons and cell bodies has not cell protein (6) . The exact ultrastructural localizabeen ascertained . tion of such a large amount of actin-like material We have used an established line of murine neuis essential in order to interpret its function in the roblastoma cells which are capable of differentiatmotile processes of nerve cells . The in situ distribuing into neurons when placed upon substrates . tion of actin-like fibers in glycerinated models of The axons formed by the cells may reach several nonmuscle cells was first demonstrated by the millimeters in length and contain 250-A microelegant experiments of Ishikawa et al . (12) . This tubules, 100-A filaments, and dense-core granules technique was based on Huxley's (11) demon(15, 20, 21 ; J . Rosenbaum, personal communicastration that F-actin forms characteristic arrowtion) . Other nervelike properties of these cells inhead complexes (decorated filaments) when clude their ability to generate action potentials in treated with heavy meromyosin (HMM) . Since response to acetylcholine or electrical stimulation then other investigators have utilized this tech(10, 14) and their production of enzymes which nique to demonstrate the existence of actin-like synthesize and metabolize neurotransmitters (2, components in a variety of cell types (1, 4, 7, 9, 3, 17) . In this report, we describe a shorter glyceri16) . Chick embryonic nerve cells contain actin-like nation procedure which preserves overall nerve fibers which interact with HMM (12) ; however, cell shape and thus permits the ultrastructural

THE LOCALIZATION OF ACTIN-LIKE FIBERS IN CULTURED NEUROBLASTOMA CELLS AS REVEALED BY HEAVY MEROMYOSIN BINDING