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Golgi bodies in amebae depend upon the nucleus for maintenance of their normal form and numbers. They decline in size and number following enucleation and appear to be absent from cells enucleated for longer than 3 days (Flickinger, 1968) . If a new nucleus is introduced by nuclear transfer into an ameba previously enucleated for 5 days, Golgi bodies are regenerated within hours (Flickinger, 1969) . Thus, the formation of many Golgi bodies can be brought about rapidly and reproducibly by experimental manipulation . This system can be used to study some of the requirements for the formation of Golgi bodies by exposing renucleated amebae to metabolic inhibitors . The size and number of Golgi bodies formed in the presence of an inhibitor are compared with the size and number of those formed in untreated renucleated amebae . In the present study, the effects of inhibitors of RNA and protein synthesis upon the formation of Golgi bodies are reported .

DECREASED FORMATION OF GOLGI BODIES IN AMEBAE IN THE PRESENCE OF RNA AND PROTEIN SYNTHESIS INHIBITORS