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Laser micro-irradiation of cell inclusions has become a useful method in cell biology (1). A pulsed ruby laser microbeam system has been successfully used to study morphological and functional relationships within mitochondria of tissue culture cells vitally stained with Janus green B (2-6) . Recently, an argon ion gas laser microbeam has been used to place lesions on mitotic chromosomes of salamander lung cells in tissue culture; these cells were photosensitized by short pretreatment (5 min) incubation with a dilute (2 X 10's%o w/v) solution of acridine orange (7, 8) . The same argon system is also being used to selectively place lesions in the large sarcosomes (mitochondria) of beating rat myocardial cells in tissue culture without prior pretreatment with a photosensitizing agent (unpublished data) . Generally, the rapid pulse repetition rate and/or continuous wave capabilities, multiple or single wavelength capacity, wide power and energy range, wavelengths in the biologically active region of the spectrum, and the existence

ARGON LASER MICRO-IRRADIATION OF NUCLEOLI