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Chang liver cells, from a suspension culture of a cell strain originally derived from liver (2), show a decrease in the rate of incorporation of labeled precursors into protein and nucleic acids when maintained in a glutamine-deficient medium (3) . The rate of incorporation of labeled amino acids decreases to 20-30% of the rate in exponentially growing cells . 2-4 hr after the addition of glutamine, protein synthesis is restored to a rate comparable to that of normally growing cells (3) . It is of interest, then, to examine normally growing and glutamine-deprived cells to see what, if any, ultrastructural changes are associated with the decreased rate of protein synthesis in glutamine deprived cells .

REVERSIBLE CHANGES IN THE ULTRASTRUCTURE OF CHANG LIVER CELL MITOCHONDRIA FOLLOWING INCUBATION OF THE CELLS IN A GLUTAMINE-DEFICIENT MEDIUM