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In the process of evaluating various fixatives for perfusion fixation of the central nervous system we have found that the neuronal microtubules are singularly sensitive to the perfusate used. In our experience the use of bicarbonate buffer results in a disintegration of neuronal microtubules; surprisingly, acrolein fixation alone uncombined with any other aldehyde also causes a similar loss. This finding is particularly noteworthy because other morphological features can remain apparently unchanged and well preserved.

MICROTUBULE LOSS WITH ACROLEIN AND BICARBONATE-CONTAINING FIXATIVES