Open AccessMolecular characterization of a cell wall-associated ß(1-3)endoglucanase ofAspergillus fumigatus
Author(s) -
Isabelle Mouyna,
J. Sarfati,
P. Recco,
Thierry Fontaine,
Bernard Henrissat,
JeanPaul Latgé
Publication year - 2002
Publication title -
medical mycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.004
H-Index - 86
eISSN - 1460-2709
pISSN - 1369-3786
DOI - 10.1080/mmy.40.5.455.464
Subject(s) - aspergillus fumigatus , cell wall , open reading frame , cellulase , gene , biology , microbiology and biotechnology , peptide sequence , aspergillus , molecular cloning , cloning (programming) , biochemistry , enzyme , programming language , computer science
A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
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