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The impact of the six-again rule change on the movement of National Rugby League (NRL) athletes was examined. Player Global Navigation Satellite System (GNSS) data (10 Hz) was collected from 42 athletes who competed in 56 matches across the 2019 to 2021 NRL seasons. Maximal mean speed (m·min −1 ) and acceleration (m·s −2 ) were established across a 10 s to 10-min duration via raw GNSS files, with subsequent intercept (mean estimates) and slope values determined via power law analysis. The distributions of match distance (m) and impulse (kN·s −1 ) were established during ball-in-play time. To determine the significance between positions and seasons under different rules, linear mixed models were used. Effects were described using standardised effect sizes (ES) with 90% confidence limits (CL). Acceleration intercepts (power law-derived) across all positions were substantially greater (&gt;0.6 SD) following the introduction of the six-again rule in the 2020 (mean ± SD; 1.02 ± 0.10 m·s −2 ) and 2021 seasons (1.05 ± 0.08 m·s −2 ) compared to the 2019 season (0.91 ± 0.07 m·s −2 ). Mean acceleration during ball-in-play time was greater in 2020 (ES; 90% CL = 0.75; ± 0.32) compared to 2019. The acceleration requirements of rugby league increased across all positional groups following the modification in NRL competition rules. Practitioners should tailor training programs for athletes to reflect the increased acceleration intensity found under the revised competition format.

The introduction of the six-again rule has increased acceleration intensity across all positions in the National Rugby League competition