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In the current study, zinc oxide (ZnO) nanoparticles were green synthesized using  Carica papaya  leaf extract and tested for larvicidal activity against  Anopheles culicifacies . The  C. papaya  ZnO nanoparticles (CP-ZnONPs) were characterized using UV–Visible spectrophotometer, FTIR, XRD, and TEM analysis. The hemolytic and antioxidant assays were also performed for the estimation of toxicity and the antioxidant potential of the CP-ZnONPs. The synthesized CP-ZnONPs were crystalline in nature with an average size of 20 nm. The observed LC 50  and LC 90  values against  A. culicifacies  larvae after 48 h of exposure were 92.17 ± 1.02 and 316.66 ± 1.24 µg/mL. CP-ZnONPs offered larval mortality of and 96.36 ± 1.15% after 48 h at 500 µg/mL. Free radical inhibition of 58.02% was observed at the highest tested concentration (100 mg/mL). The results strongly endorse the development of CP-ZnONPs as novel larvicidal agents.

Larvicidal activity of green synthesized zinc oxide nanoparticles from Carica papaya leaf extract