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The synthesis of metal nanoparticles by a green method is eco-friendly, nontoxic and few benefits to the growing fields of research used inhibit bacteria and cancer growth over the recent years. In this study, CuO NPs are synthesized through  Euphorbia hirta  leaf extract. Formation of CuO NPs was confirmed by SPR peak at UV spectrum. FTIR identified functional groups are responsible for reduction and stabilization, GC–MS, phytochemicals present in the  Euphorbia hirta  extract accountable for synthesizing CuO NPs. XRD confirmed crystalline nature, SEM and TEM confirmed size and spherical morphology. Furthermore, CuO NPs effective antibacterial activity to inhibit pathogens, strong biological activities regarding antioxidant activity and anti-inflammatory activity and excellent anticancer activity against the Ht-29 cell line. Also, good catalyst for the effective degradation of methylene blue. The present study suggests that biosynthesized CuO NPs through an extract from  Euphorbia hirta  may be used for therapeutic application in future clinical studies.

Green synthesis of copper oxide nanoparticles using Euphorbia hirta leaves extract and its biological applications