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In the present study, we have sequenced, annotated and characterized the complete mitogenome of  Tanichthys flavianalis  Li, Liao &amp; Shen, 2022, which is an endemic minnow to Hainan Province, China. The entire mitogenome size is 16,545 bp, containing 37 genes coding for 13 mitochondrial proteins, 22 tRNAs, 2 rRNAs, and one putative control region. It has the typical fish mitochondrial gene arrangement, though with 9 gene overlaps and 17 intergenic spacers scattering throughout the mitogenome, and a high A + T bias (60.8%) comparing to other Cyprinids. ATG is used as the start codon in most mitochondrial protein coding genes (PCGs) except for  cox1  which uses GTG, whereas the canonical TAA/TAG are prevailing stop codons except for  cox2 ,  cox3 ,  nad4  and  cytb . Phylogenetic analyses with other reported species from Tanichyidae were conducted using IQ-TREE and MrBayes, based on the concatenated 13 PCGs. The results clustered  T. flavinalis  with the newly described  T. albiventris  Li Bohlen &amp; Liao, 2022, and proved the reciprocally monophyletic relationship between Chinese and Vietnamese  Tanichthys .

The complete mitochondrial genome of the Tanichthys flavianalis (Cypriniformes: Tanichthyidae) and its phylogeny