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Semiothisa cinerearia  Bremer &amp; Grey, 1853 belongs to the lepidopteran family Geometridae, subfamily Ennominae. We sequenced the complete mitochondrial genome of  S. cinerearia  by PCR and Sanger sequencing method. The mitochondrial genome of  S. cinerearia  is 15,523 bp in length and contains a typical set of 37 genes with ‘MIQ’ type gene arrangement and a 394 bp AT-rich regions. Except for  cox1  using CGA as initiation codon, all protein-coding genes (PCGs) start with ATN codons and except for  nad2  and  nad4l  using TAG as termination codon, all PCGs terminated with TAA codon. A phylogenetic tree including 39 genus of subfamily Ennominae was first reconstructed based on the mitochondrial genome sequences with nucleotide substitution model GTR + G + I, which showed that the genera  Amraica ,  Jankowskia , and  Ectropis  are not monophyletic and  S. cinerearia  and  Macaria notata  are classified together.

mitochondrial dna part b

The complete mitochondrial genome of  Semiothisa cinerearia  Bremer &amp; Grey 1853 (Lepidoptera: Geometridae: Ennominae) and its phylogenetic analysis