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Scutellaria barbata  D. Don 1825 is an important medicinal plant distributed in wetlands about 2000 m above sea level and used to treat various diseases. The complete chloroplast genome of  S. barbata  is 152,050 bp with four subregions consisting of a large single-copy region (84,053 bp), a small single-copy region (17,517 bp), and a pair of inverted repeats (25,240 bp). In the chloroplast genome of  S. barbata , 131 genes were detected, comprising 87 protein-encoding genes, eight ribosomal RNA (rRNA) genes, and 36 transfer RNA (tRNA) genes. Phylogenetic analysis based on the complete chloroplast genome and protein-coding DNA sequences of 27 related taxa of the genus (out group included  Holmskioldia sanguinea  and  Tinnea aethiopica ) indicates that  S. barbata  was made a clade with  S. orthocalyx , and  S. meehanioides  was a sister to them. The first chloroplast genome of  S. barbata  was reported in this work, serving as a potential reference for important medicinal plants within the  Scutellaria  genus.

The complete chloroplast genome of Scutellaria barbata D. Don 1825 revealed the phylogenetic relationships of the Scutellaria genus