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In this study, we assembled the complete chloroplast (cp) genome of  Cynanchum acutum  subsp.  sibiricum  using high-throughput Illumina sequencing reads. The resulting chloroplast genome assembly displayed a typical quadripartite structure with a total length of 158,283 bp, which contained a pair of inverted repeat regions (IRs) of 24,459 bp. These two IRs were separated by a large single-copy region (LSC) and a small single-copy region (SSC) of 89,424 bp and 19,941 bp in length, respectively. The  C. acutum  subsp.  sibiricum  cp genome contained 130 genes, and its overall GC content was 37.87%. Phylogenetic analysis among  C. acutum  subsp.  sibiricum  and nine other  Cynanchum  species demonstrated that  C. acutum  subsp.  sibiricum  was closely related to  C. chinense . The  C. acutum  subsp.  sibiricum  cp genome presented in this study lays a good foundation for further genetic and genomic studies of the  Cynanchum  as well as Apocynaceae.

Characterization of the complete chloroplast genome of Cynanchum acutum subsp. s ibiricum (Apocynaceae)