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For the first time, this study presents the complete mitochondrial genome and phylogenetic analysis of  Eonemachilus longidorsalis , an endemic species in southwest China. The mitogenome is a circular molecule of 16,569 bp in length with a base composition of 30.2% A, 27.3% T, 16.6% G, and 25.9% C. The phylogenetic analysis based on the complete mitogenomes shows that  E. Longidorsalis  clusters with  Yunnanilus jiuchiensis ,  Yunnanilus pleurotaenia  and  Eonemachilus niger.  This contribution may provide a valuable framework for completely resolving phylogenetic relationships of the family Nemacheilidae in future research.

Complete mitochondrial genome of Eonemachilus longidorsalis (Cypriniformes: Nemacheilidae): genome characterization and phylogenetic consideration