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Histology images are widely used to assess the microstructure of biological tissues, but scanners often save images in bulky SVS and multi-layered TIFF formats. These formats were designed to archive image blocks and high-resolution textual information and are not compatible with conventional image analysis software. Our goal was to create a freeware Histology Image Viewer and Converter (HIVC) with a graphical user interface that allows viewing and converting whole-slide images into batches. HIVC was developed using C# Language for Windows x64 operating system. HIVC’s performance was assessed by converting 20 whole-slide images to a JPG format at 20× and 40× resolution and comparing the results to ImageJ, Cell Profiler, QuPath, Nanoborb, and Aperio ImageScope. HIVC was more than 8-times faster in converting images than other software packages. This software allows high-speed batch conversion of histology images to traditional formats, permitting platform-independent secondary analyses.

Histology Image Viewer and Converter (HIVC): a high-speed freeware software to view and convert whole slide histology images