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Wheat straw is commonly used as a cellulose source in mushroom compost and could be a secondary source of mycotoxin contamination in the food chain. We cultivated edible  Agaricus bisporus  and  Pleurotus ostreatus  on T-2/HT-2 artificially-contaminated mushroom compost and developed and in-house validated an UHPLC-MS/MS method for determination of T-2, HT-2, T2-triol and T2-tetraol in mushroom compost and mushroom basidiocarp. A rapid phase I metabolization of T-2 and HT-2 in mushroom compost was observed. In  Agaricus bisporus , basidiocarps 8–15 µg kg −1  accumulation of HT-2 calculated on wet weight was measured. No detectable mycotoxins were found in  Pleurotus ostreatus  basidiocarp.

Accumulation of HT-2 toxin from contaminated mushroom compost by edible Agaricus bisporus