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Tribulus terrestris  L. contains compounds with antioxidant and anti-inflammatory properties, but its effects on exercise-induced oxidative stress and inflammatory responses are unclear. The aim of this study was to examine whether  Tribulus terrestris  L. supplementation can attenuate oxidative stress and inflammatory responses to acute aerobic exercise and improve DOMS. In a randomized, double-blind, crossover design study, thirteen healthy men received either a daily supplement of  Tribulus terrestris  L. or a placebo for 4 weeks (2-week wash-out period between trials). Before and after the supplementation periods, participants performed an exercise test to exhaustion (75% VO 2 max). DOMS, thigh girth, and knee joint range of motion (KJRM) were assessed before and after the exercise (2, 24, and 48 h). Blood samples were analyzed for reduced (GSH) and oxidized (GSSG) glutathione, GSH/GSSG ratio, protein carbonyls, total antioxidant capacity, creatine kinase activity, white blood cell count, and TBARS. Acute exercise to exhaustion induced inflammatory responses and changed the blood redox status in both Tribulus and Placebo groups ( p  &lt; 0.050).  Tribulus terrestris  L. improved GSH fall ( p  = 0.005), GSSG rise ( p  = 0.001) and maintained a higher level of GSH/GSSG ratio at the 2 h point ( p  = 0.034). TBARS were lowered, protein carbonyls, creatine kinase activity, and white blood cell count elevation diminished significantly ( p  &lt; 0.050).  Tribulus terrestris  L. administration did not affect DOMS, thigh girth, or KJRM ( p  &gt; 0.050). 4-weeks of  Tribulus terrestris  L. supplementation effectively attenuates oxidative stress responses but cannot improve DOMS.

Effect of Tribulus terrestris L. supplementation on Exercise-Induced Oxidative Stress and Delayed Onset Muscle Soreness Markers: A Pilot Study