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Lignocellulosic feedstocks for biorefinery are likely to be seasonal and the supply of feedstock to cellulosic biorefineries remains a challenge. One way to overcome this is by utilizing a mixed feedstock which facilitates the maintenance of a year-round feedstock supply. This study investigated the impact of mixing of three industrially relevant cellulosic feedstocks—wheat straw, willow and  Miscanthus  using two major performance indicators—sugar yield and fermentation inhibitor production. A microwave hydrothermal pre-treatment regime of 200 °C for 5 min was applied to each feedstock individually and to 1:1 (w/w) mixes and the predicted sugar yield in the mixes was compared to the observed values. All the mixes resulted in improved sugar yields with willow +  Miscanthus  (15.4%,  p  = 0.015) and wheat + willow (13.6%,  p  = 0.010) showing a statistically significant improvement. Saccharification kinetics, inhibitor production, impact on yeast metabolic activity and growth were compared and no adverse impacts of mixing were observed. The use of mixed feedstocks in a hot water based commercial production of biofuels is unlikely to have any adverse effects on productivity and may indeed prove beneficial.

biofuels

Impact of mixed feedstock of wheat straw, willow and  Miscanthus  on enzymatic hydrolysis and inhibitor production after microwave hydrothermal pre-treatment in Europe