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Lipases are widely used in many biochemical industries. Media optimization is considered one of the best ways to produce a large quantity of lipase economically. This study used Response Surface Methodology (D - Optimal design in MODDE 13 to comprehend the effect of different media on the enzyme activity. The enzyme activity was highest under the following conditions: 10.1 g/L of peptone, 7.5 g/L of Yeast Extract, and 13.9 mL/L of Olive oil. A feasibility analysis of lipase production from the microbes using the optimized data was performed, and at optimized conditions, lipase activity increased from 0.757 μmol/min to 0.959 μmol/min. A simulation study for large-scale batch production for lipase was conducted using SuperPro Designer (v10), and techno-economic data was analyzed. The expected cost per unit volume of lipase was estimated to be 4393.96 $. Thus, process optimization with technoeconomic analysis could be helpful for industrial-scale lipase production.

Process optimization and techno-economic analysis for the production of lipase from Bacillus sp.