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Cottonseed is potential candidate to act as an important protein source but presence of toxic compound gossypol with concentration more than 0.045% is main hurdle in utilization of this protein. Optimized lyophilized protein powder constitutes 5.05% moisture, 93.1% crude protein, 0.03% free gossypol, 0.27% total gossypol, and total bacterial count of 1295 cfu/g with no presence of pathogenic bacteria. Cottonseed may be utilized as an ingredient for food industry as a supplement in various foods since it has surpassed all critical quality standards except lysine content as mentioned in Food Safety and Standards Regulations, 2011 (FSSR, version IV, 2017).

Evaluation of detoxified cottonseed protein isolate for application as food supplement