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Although bioethanol is a suitable alternative to fossil-based fuels, limited technologies or lack of microorganisms with a broad substrate spectrum are serious bottlenecks for lignocellulosic ethanol production. In this context, the current study aimed to optimize bioethanol production from industrial tea waste by applying co-fermentation strategy for the first time. For this purpose, newly isolated xylose fermenter  Candida boidinii  and  Saccharomyces cerevisiae  produced 12.1 g/L and 14.1 g/L bioethanol, respectively, when the yeasts were cultivated alone in 20% (w/v) dilute acid pretreated and enzymatically hydrolyzed industrial tea waste. Bioethanol production increased to 21.9 g/L, and 70% improvement was achieved when the co-fermentation strategy was applied under the same conditions. This study shows that co-fermentation of industrial tea waste is an economically viable choice for value-added bioethanol production.

Saccharomyces cerevisiae and newly isolated Candida boidinii co-fermentation of industrial tea waste for improved bioethanol production